Journal: iScience

Article Title: Targeted plasma proteomics identifies MICA and IL1R1 proteins associated with HIV-1 reservoir size

doi: 10.1016/j.isci.2023.106486

Figure Lengend Snippet: Key resources table

Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Biological samples Individuals living with HIV on suppressive cART (200HIV) Radboud university medical center www.humanfunctionalgenomics.org Individuals living with HIV on suppressive cART (2000HIV study) Radboud university medical center; NCT03994835 www.humanfunctionalgenomics.org Individuals living with HIV and healthy individuals without HIV (2000hiv-trained) Radboud university medical center; NCT04968717 N/A Critical commercial assays EasySep Human CD4+ T cell Isolation Kit STEMCELL 17952 DNeasy Blood & Tissue kit QIAGEN 69506 Innuprep RNA kit westburg AJ 845-KS2040250 qScript cDNA SuperMix Quantabio 95048-100 Olink® Explore panels Olink Proteomics Olink® Explore 1536 Human MICA DuoSet ELISA kit R&D Systems Cat#DY1300 PAXgene Blood RNA kit QIAGEN 762174 iScript cDNA synthesis kit Bio-Rad 1708891 SYBR Green power mix Life Technologies 4368708 Deposited data QTL summary statistics Zhang et al. 10 N/A Proteomic data with age group and sex as metadata This work https://doi.org/10.6084/m9.figshare.22058945 Oligonucleotides Primers for RPL37A , IL1R1 , MICA and FETUB , see Table S1 This paper N/A Software and algorithms R version 4.02 R core team https://cran.r-project.org Limma package version 3.48.3 Ritchie et al. 48 http://bioinf.wehi.edu.au/limma Ggplot2 package version 3.3.6 N/A https://ggplot2.tidyverse.org Ggbiplot package version 0.55 N/A http://github.com/vqv/ggbiplot Ggpubr package version 0.4.0 N/A https://rpkgs.datanovia.com/ggpubr/ R script used for analysis This work https://github.com/Blaauwmarc/Plasma-proteomics-and-HIV-viral-reservoir Open in a separate window Key resources table .

Techniques: Cell Isolation, Enzyme-linked Immunosorbent Assay, SYBR Green Assay, Software